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tricine polyacrylamide gels  (Thermo Fisher)


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    Thermo Fisher tricine polyacrylamide gels
    Tricine Polyacrylamide Gels, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cathode+buffer/TRIS-Tricine-SDS+running+buffer+(10X)%2C+cathode+buffer%2C+pH+8%2E3/pm42218136-299-8-11
    Average 95 stars, based on 1 article reviews
    tricine polyacrylamide gels - by Bioz Stars, 2026-09
    95/100 stars

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    Nucleic Acid Electrophoresis:

    Article Title: Phosphorylation regulates arginine-rich RNA-binding protein solubility and oligomerization
    Article Snippet: .. Samples and Blue Native Protein Ladder (ThermoFisher LC0725) were loaded onto NativePAGE Bis-Tris Gels (3–12%, Invitrogen BN1001BOX), and gel electrophoresis was performed using anode NativePAGE Running Buffer (Invitrogen BN2001) and cathode buffer (Invitrogen BN2002) with additive (Invitrogen BN2004) and run for 15 min at 150V. .. The Dark Blue Cathode Buffer was then interchanged with Light Blue Cathode Buffer, proceeding for another 90 min at 150 V. Following this, the gel was gently rocked in 50 mM Tris-HCl, pH 7.5, and 1% SDS for 30 min and transferred using the semidry iBolt transfer system (Invitrogen) with PVDF membrane (Invitrogen IB24002) for 7 min at 20 V. Following transfer, the PVDF membrane was rocked in 8% acetic acid solution for 5 min, rinsed with distilled water, air dried, then rinsed with methanol.

    Electrophoresis:

    Article Title: Structural and biophysical properties of FopA, a major outer membrane protein of Francisella tularensis
    Article Snippet: .. Electrophoresis was performed at 150 V, 10 mA using Anode Buffer (25 mM imidazole, pH 7.0) and Cathode Buffer (0.05% sodium deoxycholate, 0.01% βDDM, 50 mM Tricine, 7.5 mM imidazole, pH 7.0) in the electrophoresis chamber system (Invitrogen, Life Technologies) at 4°C. ..

    Article Title: Structural and biophysical properties of FopA, a major outer membrane protein of Francisella tularensis
    Article Snippet: .. Electrophoresis was performed at 150 V, 10 mA using Anode Buffer (25 mM imidazole, pH 7.0) and Cathode Buffer (0.05% sodium deoxycholate, 0.01% βDDM, 50 mM Tricine, 7.5 mM imidazole, pH 7.0) in the electrophoresis chamber system (Invitrogen, Life Technologies) at 4°C. ..

    Article Title: Indispensable role of Nectin‐like 4 in regulating synapse‐related molecules, synaptic structure, and individual behavior
    Article Snippet: 1The State Key Laboratory of Medical Molecular Biology, Neuroscience Center, Medical Primates Research Center, Department of Molecular Biology and Biochemistry, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Beijing, China 2Chinese Institute for Brain Research, Beijing, China 3National Human Diseases Animal Model Resource Center, Beijing Engineering Research Center for Experimental Animal Models of Human Critical Diseases, Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, China

    Concentration Assay:

    Article Title: Indispensable role of Nectin‐like 4 in regulating synapse‐related molecules, synaptic structure, and individual behavior
    Article Snippet: 1The State Key Laboratory of Medical Molecular Biology, Neuroscience Center, Medical Primates Research Center, Department of Molecular Biology and Biochemistry, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Beijing, China 2Chinese Institute for Brain Research, Beijing, China 3National Human Diseases Animal Model Resource Center, Beijing Engineering Research Center for Experimental Animal Models of Human Critical Diseases, Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, China

    Western Blot:

    Article Title: Indispensable role of Nectin‐like 4 in regulating synapse‐related molecules, synaptic structure, and individual behavior
    Article Snippet: 1The State Key Laboratory of Medical Molecular Biology, Neuroscience Center, Medical Primates Research Center, Department of Molecular Biology and Biochemistry, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Beijing, China 2Chinese Institute for Brain Research, Beijing, China 3National Human Diseases Animal Model Resource Center, Beijing Engineering Research Center for Experimental Animal Models of Human Critical Diseases, Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, China



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    APOE3 astrocytes have higher levels of APOE and higher levels of large APOE particles at baseline and upon NPC1 inhibition. Three isogenic APOE3 and APOE4 hiPSC-derived neuron−astrocyte (iN + hAs) cocultures were maintained for 1 week and treated with either the Veh (PBS) or the NPC1-inhibitor U18666A (NPC1 (−); 10 µg/mL) for 3 days. A) Representative <t>native</t> <t>PAGE</t> followed by APOE western blot showing large and small APOE particles in the culture media. B) Quantification of large and small APOE particle band intensity from native PAGE blots. C) Total APOE levels measured in culture media using an APOE ELISA assay. D) Representative western blot showing total APOE levels in iN + hAs lysates. E) Quantification of APOE band intensity from western blots. APOE levels were normalized to total protein levels. Data for each condition were averaged from at least three independent experiments (full differentiation of all isogenic lines) per isogenic line in B and C, and from two independent experiments per isogenic line in E. Each data point represents an isogenic line in B, C, and E. Statistical analysis was performed using two-way ANOVA with pairwise matching. Significant differences ( P < 0.05) are indicated on the graphs.
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    APOE3 astrocytes have higher levels of APOE and higher levels of large APOE particles at baseline and upon NPC1 inhibition. Three isogenic APOE3 and APOE4 hiPSC-derived neuron−astrocyte (iN + hAs) cocultures were maintained for 1 week and treated with either the Veh (PBS) or the NPC1-inhibitor U18666A (NPC1 (−); 10 µg/mL) for 3 days. A) Representative native PAGE followed by APOE western blot showing large and small APOE particles in the culture media. B) Quantification of large and small APOE particle band intensity from native PAGE blots. C) Total APOE levels measured in culture media using an APOE ELISA assay. D) Representative western blot showing total APOE levels in iN + hAs lysates. E) Quantification of APOE band intensity from western blots. APOE levels were normalized to total protein levels. Data for each condition were averaged from at least three independent experiments (full differentiation of all isogenic lines) per isogenic line in B and C, and from two independent experiments per isogenic line in E. Each data point represents an isogenic line in B, C, and E. Statistical analysis was performed using two-way ANOVA with pairwise matching. Significant differences ( P < 0.05) are indicated on the graphs.

    Journal: PNAS Nexus

    Article Title: APOE3 astrocytes can rescue lipid abnormalities and dystrophic neurites of APOE4 human neurons

    doi: 10.1093/pnasnexus/pgag053

    Figure Lengend Snippet: APOE3 astrocytes have higher levels of APOE and higher levels of large APOE particles at baseline and upon NPC1 inhibition. Three isogenic APOE3 and APOE4 hiPSC-derived neuron−astrocyte (iN + hAs) cocultures were maintained for 1 week and treated with either the Veh (PBS) or the NPC1-inhibitor U18666A (NPC1 (−); 10 µg/mL) for 3 days. A) Representative native PAGE followed by APOE western blot showing large and small APOE particles in the culture media. B) Quantification of large and small APOE particle band intensity from native PAGE blots. C) Total APOE levels measured in culture media using an APOE ELISA assay. D) Representative western blot showing total APOE levels in iN + hAs lysates. E) Quantification of APOE band intensity from western blots. APOE levels were normalized to total protein levels. Data for each condition were averaged from at least three independent experiments (full differentiation of all isogenic lines) per isogenic line in B and C, and from two independent experiments per isogenic line in E. Each data point represents an isogenic line in B, C, and E. Statistical analysis was performed using two-way ANOVA with pairwise matching. Significant differences ( P < 0.05) are indicated on the graphs.

    Article Snippet: Media samples were mixed with Native Sample Buffer (Bio-Rad, #1610738) supplemented with G-250 Sample Additive (Invitrogen, #BN2004) and run on 4–20% polyacrylamide tris-glycine gels in the absence of sodium dodecyl sulfate, reducing agents or sample boiling, in tris-glycine buffer supplemented with 1:20 native PAGE Cathode Buffer Additive (Invitrogen, #BN2002), at 150 V for 15 min.

    Techniques: Inhibition, Derivative Assay, Clear Native PAGE, Western Blot, Enzyme-linked Immunosorbent Assay